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The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling

The Tg K14-CTSL mice are viable, fertile and did not show a skin phenotype.

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The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling

E RT-PCR endpoint amplification; 50 cycles for detection of CTSL and GAPDH mRNA in primary dermal fibroblasts and epidermal keratinocytes.

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Reverse transcription for the generation of cDNA from total RNA was performed by using a first-strand cDNA synthesis kit Invitrogen, Karlsruhe, Germany.

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The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling

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The release of 7-amino-4-methyl-coumarin was continuously monitored for 1 hour by spectrofluorometry at excitation and emission wavelengths of 360 nm and 460 nm, respectively.

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It has also been shown that EGF is degraded in hepatocytes by cathepsin B, which is another representative of the papain-like cysteine peptidases.

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